Archival May-Grünwald–Giemsa-Stained Bone Marrow Smears Are an Eligible Source for Molecular DNA Research
نویسندگان
چکیده
منابع مشابه
Paediatric leukaemia DNA methylation profiling using MBD enrichment and SOLiD sequencing on archival bone marrow smears
BACKGROUND Acute Lymphoblastic Leukaemia (ALL) is the most common cancer in children. Over the past four decades, research has advanced the treatment of this cancer from a less than 60% chance of survival to over 85% today. The causal molecular mechanisms remain unclear. Here, we performed sequencing-based genomic DNA methylation profiling of eight paediatric ALL patients using archived bone ma...
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DNA from archival Papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human beta-globin, human beta-actin and human papillomavirus L1 genes. Only negligible differences in amplification efficiency were obse...
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Histochemical procedures for PMN granule enzymes were carried out on smears prepared from normal rabbit bone marrow, and the smears were examined by light microscopy. For each of the enzymes tested, azo dye and heavy metal techniques were utilized when possible. The distribution and intensity of each reaction were compared to the distribution of azurophil and specific granules in developing PMN...
متن کاملLymphoglandular bodies in bone marrow aspirate smears
which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. A 60-year-old man presented with fever for 2 weeks. Ultrasonography and contrast-enhanced abdominal computed tomography revealed retroperitoneal and mesenteric lymphadenopathy. Fine-needle aspiration cytology of a right inguinal lymph node showed a monotonou...
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Background. FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow...
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ژورنال
عنوان ژورنال: Biopreservation and Biobanking
سال: 2019
ISSN: 1947-5535,1947-5543
DOI: 10.1089/bio.2018.0062